UV-Vis Absorbance Microplate Reader with Monochromator, 200–1000 nm
Quick Overview
The FlexA-200 Series is a UV-Vis absorbance microplate reader featuring a monochromator-based optical system. It supports 96- and 384-well microplates and enables wavelength selection from 200 to 1000 nm in 1 nm increments without filter changes.
The instrument supports endpoint, kinetic, and full-spectrum scanning measurements. A xenon flash lamp, dual photodetectors, and automatic optical calibration ensure stable absorbance data. The 10-inch touchscreen and built-in software allow standalone operation without a computer.
Available models: FlexA-200 and FlexA-200HT
Detection mode: UV-Vis absorbance
Wavelength range: 200-1000 nm
Supported plates: 96- and 384-well microplates
Measurement modes: Endpoint, kinetic and spectral scanning
Incubation: Room temperature +4°C to 45°C
Product Description
The FlexA-200 and FlexA-200HT are monochromator-based absorbance microplate readers designed for flexible UV-Vis measurements in life science, pharmaceutical, and biotechnology research.
The xenon flash lamp and grating monochromator allow wavelength selection from 200 to 1000 nm in 1 nm increments without optical filter replacement. Full-spectrum scanning helps identify optimal measurement wavelengths for new assays.
The dual-beam optical system employs two silicon photodetectors: one for sample measurement and one as reference. Automatic system checks at startup ensure stable operation.
The instrument reads a 96-well plate in under 8 seconds in fast endpoint mode or under 28 seconds in accurate mode. The incubation system operates from room temperature plus 4°C to 45°C, with linear shaking at three adjustable speeds for mixing and temperature-controlled kinetic assays.
A 10-inch touchscreen and built-in Android software enable plate layout creation, standard curves, and measurement programming directly on the instrument. Results export in Excel, TXT, CSV, or PDF formats, with ReaderIt-II PC software and FTP data transfer support.
The FlexA-200HT includes an independent cuvette slot with 200-1000 nm wavelength range and temperature-controlled incubation, supporting endpoint, kinetic, spectral scanning, and standard-curve measurements.
An optional u-Nano ultra-micro plate is available for low-volume nucleic acid and protein quantification.
Applications
- ELISA absorbance assays
- DNA and RNA quantification
- Protein concentration measurement
- Enzyme kinetics
- Cell growth and proliferation assays
- Colorimetric assays
- Standard curve analysis
- Endpoint absorbance measurements
- Kinetic absorbance measurements
- UV-Vis spectral scanning
- Microbial growth monitoring
- Pharmaceutical and biotechnology research
- Low-volume nucleic acid and protein analysis with optional u-Nano plate
- Cuvette-based UV-Vis analysis (FlexA-200HT only)
The series is suitable for universities, research institutes, biotechnology companies, and pharmaceutical research laboratories. Cuvette measurements are available only with the FlexA-200HT model.
Technical Specifications
| Parameter |
FlexA-200 |
FlexA-200HT |
| Detection Mode |
UV-Vis absorbance |
UV-Vis absorbance |
| Supported Microplates |
96- and 384-well plates |
96- and 384-well plates |
| Display |
10-inch high-resolution capacitive touchscreen |
Same |
| Light Source |
Xenon flash lamp, ＞10⁹ flashes |
Same |
| Wavelength Range |
200-1000 nm |
Same |
| Wavelength Accuracy |
2 nm |
Same |
| Wavelength Repeatability |
0.2 nm |
Same |
| Optical System |
Monochromator, 1 nm step |
Same |
| Reading Range |
0-4.0 OD |
Same |
| Bandwidth |
＜2.5 nm |
Same |
| Detection System |
2 silicon photodetectors: one for measurement and one for reference |
Same |
| Linearity at 450 nm |
R² ≥ 0.999 at 0.0-3.0 Abs |
Same |
| Absorbance Accuracy at 450 nm |
±(1.0% + 0.003 Abs) at 0-2.0 Abs; ±2.0% at 2.0-2.5 Abs |
Same |
| Absorbance Repeatability at 450 nm |
Accurate mode: CV＜0.5% or SD＜0.003; fast mode: CV＜1.0% |
Same |
| Measuring Speed |
96-well plate: fast mode ＜8 s; accurate mode ＜28 s, endpoint method |
Same |
| Measurement Modes |
Endpoint, kinetic and spectral scanning |
Same |
| Microplate Shaking |
Linear, 3 adjustable speeds |
Same |
| Incubation Temperature |
Room temperature +4°C to 45°C |
Same |
| Temperature Accuracy |
±0.5°C at 37°C |
Same |
| Temperature Uniformity |
±0.5°C at 37°C |
Same |
| User Interface |
Built-in software, independent operation |
Same |
| Analysis Software |
ReaderIt-II software |
Same |
| Operation System |
Android system |
Same |
| Input Method |
Touchscreen, keyboard and mouse |
Same |
| Internal Storage |
16 GB; stores more than 20,000 data files |
Same |
| Data Export Formats |
Excel, TXT, CSV and PDF |
Same |
| Ports |
1 × USB Type-B, 2 × USB Type-A and 1 × Ethernet port |
Same |
| Robotic Arm Compatibility |
Temporarily incompatible |
Same |
| Power Supply |
DC 24 V, 6.67 A |
Same |
| Dimensions |
300 × 500 × 260 mm |
Same |
| Net Weight |
15.5 kg |
Same |
| Independent Cuvette Slot |
No |
Yes |
| Cuvette Wavelength Range |
-- |
200-1000 nm |
| Cuvette Incubation |
-- |
Room temperature +4°C to 45°C |
| Cuvette Measurement Modes |
-- |
Endpoint, kinetic, spectral scanning and standard curve |
| Recommended Use |
Microplate absorbance assays |
Microplate and cuvette absorbance assays |
